We will discuss basics of solution and solid-state NMR methods for application of structural studies of amyloid fibrils. Chemical shift assignments are often complicated in these non-crystalline aggregates and standard triple resonance methodologies often have to be supplemented with specific isotopic labeling. Residual dipolar coupling and paramagnetic enhancement measurements can be useful as additional structural constraints in these systems. Further, we will discuss sample preparation strategies and assessment of sample quality which ultimately can be a determining factor in the success of the NMR measurements.
Contact:
Prof. Dr. Björn Corzilius (host scientist)
Institute of Chemistry
Department of Physical Chemistry
Dr.-Lorenz-Weg 2
18059 Rostock